Composite

Part:BBa_K1197005

Designed by: Emre İlpars   Group: iGEM13_METU_Turkey   (2013-09-22)

MazF +RBS (SpoVG) + LacI + DT

MazF is a non-specific ribonuclease coding gene is used as killing agent against Bacillus subtilis. In our system, MazF is produced constitutively under Pveg promoter.

When MazF construct (Pveg + RBS SpoVG + MazF + RBS SpoVG + LacI + DT) was assemled in B.subtilis integration vector, BBa_K823023, we saw colonies when it is transformed into E.coli with Ampicillin resistance. On the other hand, we did not see any colony when the same plasmid was transformed into B.subtilis. Thus, it can be said that MazF construct is working in B.subtilis functionally.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Gel electrophoresis photo of MazF construct:

MazF_%2B_RBS_SpoVG_%2B_LacI_%2B_DT.jpg


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None